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Mendelian Genetics & Punnett Square Lab

Investigate inheritance patterns using Punnett squares. Cross parents with different genotypes, explore dominance patterns, analyze sex-linked traits, and compare predicted ratios to Mendel's classical results.

Guided Experiment: Monohybrid Cross and Dominance Patterns

What phenotypic ratio do you expect from crossing two heterozygous parents (Aa × Aa) under complete dominance? How does the ratio change under incomplete dominance?

Write your hypothesis in the Lab Report panel, then click Next.

Punnett Square

AaAaAADominantAaDominantAaDominantaaRecessive
Dominant(3/4 = 75.0%)
Recessive(1/4 = 25.0%)

Controls

Parent Genotypes
Dominance Pattern

Monohybrid Cross Results

Genotypic Ratio
AA:Aa:aa = 1:2:1
AA
1/4 (25.0%)
Aa
2/4 (50.0%)
aa
1/4 (25.0%)
Phenotypic Ratio
Dominant:Recessive = 3:1
Dominant
3/4 (75.0%)
Recessive
1/4 (25.0%)
Offspring Probabilities
Dominant
75.0%
3/4
Recessive
25.0%
1/4
Hardy-Weinberg Reference
p2+2pq+q2=1p^2 + 2pq + q^2 = 1

Where p = frequency of dominant allele, q = frequency of recessive allele

Data Table

(0 rows)
#Cross TypeParentsGenotypic RatioPhenotypic RatioDominant %Recessive %
0 / 500
0 / 500
0 / 500

Reference Guide

Monohybrid Cross

A monohybrid cross involves a single gene with two alleles. The heterozygous cross Aa × Aa produces offspring in a predictable ratio.

Genotypic ratio: 1AA:2Aa:1aa\text{Genotypic ratio: } 1\,AA : 2\,Aa : 1\,aa
Phenotypic ratio: 3 dominant:1 recessive\text{Phenotypic ratio: } 3 \text{ dominant} : 1 \text{ recessive}

A testcross (Aa × aa) reveals the genotype of an unknown parent by producing a 1:1 phenotypic ratio.

Dihybrid Cross

A dihybrid cross tracks two genes simultaneously. When both parents are double heterozygotes (AaBb × AaBb), independent assortment produces a 4×4 Punnett square with 16 cells.

9  A_B_:3  A_bb:3  aaB_:1  aabb9\;A\_B\_ : 3\;A\_bb : 3\;aaB\_ : 1\;aabb

Deviations from the 9:3:3:1 ratio may indicate gene linkage, epistasis, or other non-Mendelian patterns.

Incomplete & Codominance

Incomplete dominance produces a blended intermediate phenotype in heterozygotes. Example: red × white snapdragons produce pink offspring.

Phenotypic ratio: 1:2:1\text{Phenotypic ratio: } 1 : 2 : 1

Codominance means both alleles are fully expressed simultaneously. Example: blood type where IᴬIᴮ individuals express both A and B antigens.

In both cases, the genotypic ratio (1:2:1) matches the phenotypic ratio, unlike complete dominance.

Sex-Linked Traits

Sex-linked (X-linked) traits are carried on the X chromosome. Males (XY) express the trait with only one copy of the recessive allele, while females need two copies.

XAXa×XAYX^A X^a \times X^A Y

A carrier mother (XᴬXᵃ) crossed with an unaffected father (XᴬY) produces 25% affected males, 25% carrier females, and 50% unaffected offspring.

Classic examples include color blindness and hemophilia, which appear more frequently in males because they have no second X chromosome to mask the recessive allele.